Journal: bioRxiv

Article Title: MicroRNA-195-3p as a potential regulator of hypoxic injury in HUVECs

doi: 10.1101/2023.02.23.529661

Figure Lengend Snippet: miR-195-3P inhibitor regains hypoxia-induced HUVECs proliferation rate. ( A, B ) EdU results shows the proliferation rate of each group. The cells were stained with Hoechst 33342 (5 μg/mL), and the proliferation population was analyzed (n=3). Scale bar, 50 μm. ( C ) The viabilities of cells in the different treatment groups was measured using the CCK-8 assay (n=5). ( D, E ) Representative images of cell colonies. Colony formation assays determined cell proliferation in HUVECs. **p < 0.01, ***p < 0.001 vs. the control group. ##p < 0.01, ###p < 0.001 vs. hypoxia induction for 6 h group. HUVECs, human umbilical vein endothelial cells; EdU, 5-ethynyl-2’-deoxyuridine; CCK-8, Cell Counting kit-8.

Article Snippet: HUVECS viability was assessed by Cell Counting Kit-8 (CCK-8, Dojindo, Japan) assay as described previously.

Techniques: Staining, CCK-8 Assay, Control, Cell Counting

Journal: eLife

Article Title: Control of endothelial cell polarity and sprouting angiogenesis by non-centrosomal microtubules

doi: 10.7554/eLife.33864

Figure Lengend Snippet:

Article Snippet: Commercial assay or kit , AMAXA huvecs nucleofector kit , Lonza , Lonza:VPB-1002 , .

Techniques: Cell Culture, Recombinant, Sequencing, Amplification, Software

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on HUVEC and HMEC-1 viability. Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then cell viability was determined by MTT assay. *P<0.05 vs. control group (0 µg/ml PM2.5). PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC, human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques: MTT Assay, Control

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on HUVEC and HMEC-1 apoptosis. (A) Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then cell apoptosis was assessed by Annexin V-fluorescein isothiocyanate/propidium iodide assay. (B) Quantification of cell apoptosis. *P<0.05 vs. control group (0 µg/ml PM2.5). PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques: Control

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on HUVEC and HMEC-1 migration. (A) Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then cell migration was determined by Boyden chamber assay Transwell assay. (B) Quantitative analysis of cell migration. *P<0.05 vs. control group (0 µg/ml PM2.5). PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC, human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques: Migration, Boyden Chamber Assay, Transwell Assay, Control

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on HUVEC and HMEC-1 tube formation. (A) Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then tube formation was determined by Matrigel tube formation assay. (B) Quantitative analysis of tube formation. *P<0.05 vs. control group (0 µg/ml PM2.5). PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC, human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques: Tube Formation Assay, Control

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on cell adhesion of (A) HUVEC and (B) HMEC-1 to ECM proteins. Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then the absorbance of ECM proteins was determined. *P<0.05 vs. control group. PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC, human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell; ECM, extracellular matrix; Group 1, control.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques: Control

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on HUVEC- and HMEC-1-induced ROS production. Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then ROS production was determined by 2′,7′-dichlorofluorescin diacetate assay. *P<0.05 vs. control group (0 µg/ml PM2.5). PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC, human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell; ROS, reactive oxygen species.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques: Control

Journal: Molecular Medicine Reports

Article Title: PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells

doi: 10.3892/mmr.2017.6877

Figure Lengend Snippet: Effect of PM2.5 on HUVEC- and HMEC-1-induced IL-8 and TNF-α expression. Cells were treated with 0–800 µg/ml PM2.5 for 24 h, then (A) IL-8 and (B) TNF-α expression was determined by ELISA. *P<0.05 vs. control group (0 µg/ml PM2.5). PM2.5, particulate matter with a diameter of ≤2.5 µm; HUVEC human umbilical vein endothelial cell; HMEC, human microvascular endothelial cell; IL, interleukin; TNF, tumor necrosis factor.

Article Snippet: HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Cell

Article Title: Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation

doi: 10.1016/j.cell.2022.04.005

Figure Lengend Snippet: (A) The effects of Δ9-THC on cell viability of human coronary artery endothelial cells (HCAECs), human umbilical vein endothelial cells (HUVECs), normal human cardiac fibroblasts-ventricular (NHCF-V), and human embryonic stem cell-derived cardiomyocytes (hESC-CMs). Cells were treated with increasing concentrations of Δ9-THC for 48 h, and cell viability was measured by the CellTiter-Glo luminescent cell viability assay.

Article Snippet: Human umbilical vein endothelial cells (HUVEC) , ATCC , CRL-1730.

Techniques: Derivative Assay, Cell Viability Assay

Journal: Cell

Article Title: Cannabinoid receptor 1 antagonist genistein attenuates marijuana-induced vascular inflammation

doi: 10.1016/j.cell.2022.04.005

Figure Lengend Snippet: Δ 9 -THC induces cytotoxicity in human endothelial cells

Article Snippet: Human umbilical vein endothelial cells (HUVEC) , ATCC , CRL-1730.

Techniques: Virus, Recombinant, Membrane, DNA Ligation, Luminex, RNA Sequencing, Knock-Out, Control, Plasmid Preparation, Expressing, Software, Modification, Diagnostic Assay